Coding

Part:BBa_K2524000:Design

Designed by: Holly Bowman   Group: iGEM17_Georgia_State   (2017-09-29)


Synthetic Human Chorionic Gonadotropin (HCG) Beta Subunit


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

When designing this part we used the recombinant cDNA sequence. While the same amino acids are present, the cDNA sequence is altered. To detect the activation of Factor C autocatalysis, a hCG-beta subunit sequence component was a necessary addition to our final Factor C cDNA sequence. Similar to the primers designed for Factor C, we designed a forward and a reverse complement primer with iGEM prefix and suffix to join Block 4 of Factor C and Block 5 of hCG-beta subunit together. For our idea of a detection system, we plan to use a pregnancy test to detect the recombinant hCG-beta subunit once Factor C is activated.

Source

https://www.ncbi.nlm.nih.gov/protein/26996824?report=genbank&log$=protalign&blast_rank=1&RID=PU2MZS8Z014

References